If the sample contains fewer templates, the Ct value goes high at it takes more cycles to progress the reaction.
Is the gene the same from the plasmid and the genome? The test looks for genetic sequences specific to Sars-CoV-2, the virus causing Covid-19.
At each visit, enrolled household members provided a nose and throat self-swab following instructions from the study worker.
What was recommended to me was the following: Calculate the delta ct values for each subject individually.
Many thanks, Steven Hi Steven.
Calculate the fold change from each subject, average the values, and calculate the sd and run the t-test from the fold change values.